Protein function: Inhibitor of the caspase-activated DNase (DFF40). Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. S8), indicating that Xkr8, like TMEM16F ( 5 ), promotes the. termed CPAN, DFF40, and human CAD, respectively. By contrast, apoptotic hXkr8-expressing cells (but not parental cells) stained with RO09-0198 and internalized NBD-PC and NBD-SM (fig. B, SK-N-AS cells transfected with NR siRNA or CAD siRNA number 2 were treated with 1 m STP for 6 h or left untreated (Control), and nuclear morphology was analyzed by staining the nuclei with Hoechst 33258. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Inhibitor of caspase-activated DNase (ICAD) was cleaved equally well in PLB-985 and its hXkr8 transformants after staurosporine treatment (fig. The membrane was reprobed with anti-ICAD antibody (lower panel). The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFF becomes activated when DFFA is cleaved by caspase-3. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits.

The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. To determine which form of ICAD, ICAD-L or ICAD-S, is associated with CAD, the flow-through and bound fractions were analyzed by Western blotting with the anti-ICAD antibody. Note also that in the D224E ICAD a band of 30 kDa (p30 (C-term.)) is accumulated. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. Detection with an anti-ICAD antibody reveals the correct processing of endogenous ICAD in all transfected cells when treated with STP (p11 (C-term.), middle panel). DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene.

0.5mg/ml if reconstituted with 0.2ml sterile DI water.

Icad antibody